Pervasive allele-specific regulation on RNA decay in hybrid mice

Extensive allele-specific translational regulation in hybrid mice

Translational regulation is mediated through the interaction between diffusible trans-factors and cis-elements residing within mRNA transcripts. In contrast to extensively studied transcriptional regulation, cis-regulation on translation remains underexplored. Using deep sequencing-based transcriptome and polysome profiling, we globally profiled allele-specific translational efficiency for the ...

Allele-Specific Regulation of TCR

Disease-Causing Allele-Specific Silencing by RNA Interference

Small double-stranded RNAs (dsRNAs) of approximately 21-nucleotides in size, referred to as small interfering RNA (siRNA) duplexes, can induce sequence-specific posttranscriptional gene silencing, or RNA interference (RNAi). Since chemically synthesized siRNA duplexes were found to induce RNAi in mammalian cells, RNAi has become a powerful reverse genetic tool for suppressing the expression of ...

Inference of allele specific expression levels from RNA-Seq data

Accurate allele specific expression estimation requires the availability of a diploid transcriptome, which makes it a challenging problem. Most existing methods rely on simple counting of alleles coverage at heterozygous Single Nucleotide Polymorphic sites. In this work, we present RNA-PhASE, a pipeline for Allele Specific gene and isoform Expression estimation from RNA-Seq Reads. The pipeline ...

Specific Silencing of L392V PSEN1 Mutant Allele by RNA Interference

RNA interference (RNAi) technology provides a powerful molecular tool to reduce an expression of selected genes in eukaryotic cells. Short interfering RNAs (siRNAs) are the effector molecules that trigger RNAi. Here, we describe siRNAs that discriminate between the wild type and mutant (1174 C→G) alleles of human Presenilin1 gene (PSEN1). This mutation, resulting in L392V PSEN1 variant, contrib...